Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing
نویسندگان
چکیده
منابع مشابه
Rolling Circle Amplification (RCA): an approach for quick detection and identification of fungal species
Conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. These tests often need several days or weeks to complete and are frequentlyunspecific. Molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. The Rolling CircleAmplification app...
متن کاملThe Discovery of Rolling Circle Amplification and Rolling Circle Transcription.
Nucleic acid amplification is a hugely important technology for biology and medicine. While the polymerase chain reaction (PCR) has been highly useful and effective, its reliance on heating and cooling cycles places some constraints on its utility. For example, the heating step of PCR can destroy biological molecules under investigation and heat/cool cycles are not applicable in living systems....
متن کاملrolling circle amplification (rca): an approach for quick detection and identification of fungal species
conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. these tests often need several days or weeks to complete and are frequentlyunspecific. molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. the rolling circleamplification app...
متن کاملImprovements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein
Rolling circle amplification (RCA) of plasmid or genomic DNA using random hexamers and bacteriophage phi29 DNA polymerase has become increasingly popular in the amplification of template DNA in DNA sequencing. We have found that the mutant protein of single-stranded DNA binding protein (SSB) from Thermus thermophilus (Tth) HB8 enhances the efficiency of amplification of DNA templates. In additi...
متن کاملRolling Circle Amplification (RCA): an approach for quick detection and identification of fungal species
Conventional methods for fungal identification in the clinical laboratory rely on morphological and physiological tests. These tests often need several days or weeks to complete and are frequently unspecific. Molecular identification mostly implies sequencing, which is relatively expensive and time-consuming, as well as impractical for large numbers of isolates. The Rolling Circle Amplification...
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ژورنال
عنوان ژورنال: Pharmaceuticals
سال: 2018
ISSN: 1424-8247
DOI: 10.3390/ph11020035